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Biologia
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Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry
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Biologia
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JoVE Journal Biologia
Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry

Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry

DOI:
10.3791/2686-v

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06:46 min

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June 06, 2011

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, , , , ,
1Department of Biochemistry,Albert Einstein College of Medicine, Yeshiva University, 2Macromolecular Therapeutics Development Facility,Albert Einstein College of Medicine, Yeshiva University, 3Developmental and Molecular Biology,Albert Einstein College of Medicine, Yeshiva University

Capítulos

  • 00:05Título
  • 01:58Egg Collection and Dechorionation
  • 02:34Microinjection with GDP-FucAI
  • 03:30BTTES-Cu(I)-catalyzed Click Chemistry Reaction
  • 04:34Imaging
  • 05:06In Vivo Imaging of Fucosylated Glycans During Zebrafish Embryogenesis
  • 06:11Conclusion

Summary

Tadução automática

Tadução automática

A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.

Tags

GlycansZebrafish EmbryosMetabolic LabelingBioorthogonal Click ChemistryChemical Reporter StrategyAzide-tagged MonosaccharidesAlkyne-tagged MonosaccharidesGlycan Biosynthetic MachineryCell Surface GlycoconjugatesFluorescent ProbesAffinity ProbesNoninvasive ImagingFucosylated GlycansMicroinjectionGDP-5-alkynylfucose (GDP-FucAl)Azide-conjugated FluorophoresCu(I)-catalyzed Azide-alkyne Cycloaddition (CuAAC)Confocal MicroscopySialic Acid-containing GlycansN-acetylgalactosamine-containing Glycans

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