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Eine Methode zur Ovarialfollikel Encapsulation und Kultur in einem Proteolytisch Degradable 3 Dimensional-System
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1Institute for BioNanotechnology in Advanced Medicine,Northwestern University, 2Department of Obstetrics and Gynecology,Northwestern University, Feinberg School of Medicine, 3Center for Reproductive Research,Northwestern University, 4The Robert H. Lurie Comprehensive Cancer Center,Northwestern University, 5Department of Chemical and Biological Engineering,Northwestern University
Capítulos
- 00:05Título
- 03:02Follicle Isolation
- 05:11The Drop Method
- 07:16The Parafilm Method
- 08:31Follicle Recovery from the 3D Matrix
- 10:00Alginate and Fibrin-alginate for 3D Follicle Culture in Vitro
- 11:48Conclusion
Eine neue Methode zur Ovarialfollikel Kapselung in einer 3D-Fibrin-Alginat durchdringendes Netzwerk beschrieben. Dieses System kombiniert strukturelle Unterstützung mit proteolytischen Abbau der Entwicklung von unreifen Follikeln Unterstützung, um zu reifen Eizellen zu produzieren. Diese Methode kann zur Kultur Zellaggregate angewendet werden, um Zell-Zell-Kontakte ohne Beschränkungen für die Ausweitung aufrecht zu erhalten.
Tags
Ovarian FollicleEncapsulationCultureProteolytically Degradable3 Dimensional SystemIn Vitro Follicle TechniquesHydrogelsNative Ovarian Environment3D Follicular ArchitectureCell-cell InteractionsParacrine SignalingAlginateInert Algae-derived PolysaccharideGelationCalcium IonsDevelopmental CompetenceDegradableFibrin-alginate Interpenetrating Network (FA-IPN)Dynamic Mechanical Environment
