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Fotobleken Analyses (FRAP & FLIP) om chromatine eiwit Dynamics Meet in Living Embryonale stamcellen
JoVE Journal
Biologia
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JoVE Journal Biologia
Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells
DOI:

09:18 min

June 29, 2011

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Capítulos

  • 00:05Título
  • 01:04Plating the Stem Cells
  • 02:02Transfecting the Stem Cells
  • 03:24Performing FRAP or FLIP
  • 06:10FRAP and FLIP Data Analysis
  • 07:26FRAP and FLIP Results
  • 08:52Conclusion

Summary

Tadução automática

We beschrijven fotobleken methoden, met inbegrip Fluorescence Recovery After Photobleaching (FRAP) en fluorescentie Loss In Photobleaching (FLIP) om chromatine eiwit dynamiek monitor in embryonale stamcellen (ES) cellen. Chromatine eiwit dynamiek, die wordt beschouwd als een van de middelen om chromatine plasticiteit bestuderen, wordt versterkt in de pluripotente cellen.

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