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Capítulos
- 00:00Creating channel inlet and outlet
- 00:06Introduction
- 01:27Microfabrication of microfluidic device
- 01:45Preparation of PDMS mixture
- 02:42Patterning PDMS molds
- 03:36Removing bubbles by vacuum
- 04:21Assembly of microfluidic device
- 04:55Cutting layers of microfluidic device
- 06:10Bonding the microfluidic device layers
- 08:19Coating channels with fibronectin
- 09:08Experiment: monitoring apoptosis
- 09:09Cell seeding
- 11:31Application of reagents
- 13:32Time lapse of apoptosis
Se describe un protocolo para la fabricación de dispositivos de microfluidos que puede permitir la captura de células y la cultura. En este enfoque micro modelado como ranuras dentro de los canales de microfluidos se utilizan para crear las regiones de baja tensión de corte dentro de la celda que se puede acoplar.
Tags
Microfluidic DeviceGroove PatternsCellular BehaviorMicrogrooved SubstrateFabricationPoly(dimethylsiloxane) (PDMS)Mouse Fibroblast CellsApoptosisOxidative StressAnnexin VPropidium IodidePhosphatidylserinePlasma MembraneReal-time ObservationInverted MicroscopeIncubation ChamberHigh-throughput Drug Screening
