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Imunologia e Infecção

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非手术治疗的小鼠气管内滴入肺和肺淋巴结流式细胞仪分析

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

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非手术治疗的小鼠气管内滴入肺和肺淋巴结流式细胞仪分析

DOI:

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08:01 min

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May 02, 2011

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Manira Rayamajhi, Elizabeth F. Redente, Tracy V. Condon, Mercedes Gonzalez-Juarrero, David W.H. Riches^2,4, Laurel L. Lenz⁴

¹Department of Immunology,University of Colorado School of Medicine, ²Division of Cell Biology, Department of Pediatrics,National Jewish Health , ³Department of Microbiology, Immunology, and Pathology,Colorado State University, ⁴Department of Immunology,National Jewish Health

Capítulos

00:05Título
01:32Intratracheal Injection
03:11Harvesting and Processing Lung Draining Lymph Nodes and Lungs for Flow Cytometry
06:33Representative Flow Cytometric Analyses of Lung Draining Lymph Nodes After Intratracheal Injection
07:39Conclusion

Summary

Tadução automática

我们说明进入肺部通过气管内麻醉小鼠的非手术治疗提供试验材料。这种方法允许肺暴露于细菌和病毒的病原体，细胞因子，抗体，珠子，化学品，染料。我们进一步描述采伐和肺和肺淋巴结（LDLNs），流式细胞仪检测处理。

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