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注射胰岛素和血淋巴的提取来衡量成人胰岛素敏感性果蝇</em
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Biologia
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JoVE Journal Biologia
Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster
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注射胰岛素和血淋巴的提取来衡量成人胰岛素敏感性果蝇</em

DOI:
10.3791/2722-v

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05:52 min

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June 30, 2011

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,
1Department of Biology,State University of New York, 2Allied Health Sciences,University of Connecticut

Capítulos

  • 00:00Título
  • 00:41Introduction
  • 00:54Starvation Treatment for Adult Flies
  • 01:26Insulin Injection Preparation
  • 02:48Insulin Injection Procedure
  • 03:52Hemolymph Collection and Glucose Determination
  • 04:55Representative Insulin Tolerance Response Determined by Spectrophotometry
  • 05:13Conclusion

Summary

Tadução automática

Tadução automática

保守的胰岛素信号通路在果蝇中发现<em>果蝇</em>使这个有机体建模，包括II型糖尿病的代谢紊乱的潜在工具。为此，关键是建立生理检测在成人飞周边葡萄糖处置全身胰岛素的作用，以有效衡量。

Tags

Insulin InjectionHemolymph ExtractionInsulin SensitivityDrosophila MelanogasterGlucose HomeostasisMetabolic DisordersType II DiabetesGlucose ToleranceInsulin Signaling MutantsGlucose Tolerance ResponsePeripheral Insulin Sensitivity

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