/
/
'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 02:04Y. Pestis Plate Inoculation
- 02:53Y. Pestis Liquid Media Inoculation
- 03:30Y. Pestis Outgrowth Reporter Phage Addition and Bioluminescence Detection
- 07:06Single Time Point, Expedited Detection
- 07:41Data Analysis and Representative Results
- 09:54Conclusion
A simple method for the identification of priority bacterial pathogens is to use genetically engineered reporter phage. These reporter phage, which are specific to their particular host species, are capable of rapidly transducing a bioluminescent signal response to host cells. Herein, we describe the use of reporter phage for the detection of Yersinia pestis.
Tags
Bioluminescent Reporter PhageDetectionCategory A Bacterial PathogensYersinia PestisBacillus AnthracisPlagueAnthraxBioweaponCommunicabilityRapid Clinical CourseHigh Mortality RateOutbreak DetectionMethodologiesRapid Detection And DiagnosisPublic Health MeasuresCrisis ManagementRecombinant Reporter PhageCenters For Disease Control And PreventionPhage Lysis AssaysBacterial Pathogens IdentificationPlaques FormationLaboratory-based AssaysBacterial Isolation And Cultivation
