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Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
JoVE Journal
Biologia
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JoVE Journal Biologia
Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

DOI:
10.3791/2796-v

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08:45 min

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September 07, 2011

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, ,
1Department of Biochemistry, Cellular and Molecular Biology,University of Tennessee

Capítulos

  • 00:05Título
  • 01:06Isothermal Titration Calorimetry (ITC) Sample Preparation
  • 02:34The ITC Experiment
  • 05:46ITC Data Analysis
  • 07:20Representative ITC Results
  • 08:21Conclusion

Summary

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Tadução automática

A general protocol for the use of isothermal titration calorimetry to monitor the binding thermodynamics for biological systems with moderate binding affinities is presented.

Tags

Isothermal Titration CalorimetryITCMacromolecule-ligand AffinityBinding ReactionThermodynamic PictureBiological SciencesMacromolecular InteractionsExperimental ConditionsBufferTemperatureLigand ConcentrationMacromolecule ConcentrationAccurate DeterminationSample PreparationImpuritiesITC Experiment ControlsStoichiometryAffinityEnthalpyAdditional ExperimentsBuffer ConditionsTemperature ConditionsProtocol

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