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Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
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Biologia
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JoVE Journal Biologia
Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

DOI:
10.3791/2797-v

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13:54 min

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October 10, 2011

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, ,
1Department of Genetics,University of Georgia

Capítulos

  • 00:05Título
  • 01:09Generation of a Gene-specific Riboprobe and Measurement of Specific Activity
  • 03:58Collection of Mouse Embryos
  • 05:12Hybridization of the Digoxygenin-labeled Probe to Whole Mouse Embryos
  • 10:07Antibody Detection of the Digoxygenin-labeled Riboprobe
  • 12:38Examples of Whole-mount in situ Hybridizations
  • 13:29Conclusion

Summary

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This whole mount in situ hybridization protocol discusses critical steps that ensure reproducible high quality results for gene expression studies in E8.5-E11.5 day old mouse embryos.

Tags

Whole MountIn Situ HybridizationGene Expression PatternsEmbryosProbe LabelingQuality ControlDIG-labeled RNA ProbesIn Vitro TranscriptionPCRSensitivityEndogenous MRNAFixationStorageProteinase K DigestionHybridization ConditionsPost-hybridization WashesRNase TreatmentNon-specific Probe HybridizationAP-conjugated Antibody

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