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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
JoVE Journal
Biologia
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JoVE Journal Biologia
Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
DOI:

15:32 min

August 05, 2011

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Capítulos

  • 00:05Título
  • 02:10Harvesting of Tissues
  • 03:03Preparation of Fetal Liver Cells
  • 05:01Antibody Staining for Flow Cytometry
  • 07:14Identification and Analysis of Erythroid Progenitors
  • 13:52Conclusion

Summary

Tadução automática

A flow-cytometric method for identification and molecular analysis of differentiation-stage-specific murine erythroid progenitors and precursors, directly in freshly –harvested mouse bone marrow, spleen or fetal liver. The assay relies on cell-surface markers CD71, Ter119, and cell size.

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