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Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
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Biologia
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JoVE Journal Biologia
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

DOI:
10.3791/2810-v

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13:21 min

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August 18, 2012

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, , ,
1The Davis Heart and Lung Research Institute,The Ohio State University, 2Department of Pharmacology,College of Medicine, The Ohio State University

Capítulos

  • 00:05Título
  • 00:09Introduction
  • 02:56Detection of Nitric Oxide (NO) with Culture of Bovine Aortic Endothelial Cells (BAEC)
  • 05:56Endothelial NOS (eNOS) Uncoupling Experiment using SIN-1
  • 08:12Detection of superoxide (O2–) from polymorphonuclear neutrophils (PMNs)
  • 10:20Conclusion

Summary

Tadução automática

Tadução automática

Electron paramagnetic resonance (EPR) spectroscopy was employed to detect nitric oxide from bovine aortic endothelial cells and superoxide radical anion from human neutrophils using iron (II)-N-methyl-D-glucamine dithiocarbamate, Fe(MGD)2 and 5,5-dimethyl-1-pyroroline-N-oxide, DMPO, respectively.

Tags

DetectionNitric OxideSuperoxide Radical AnionElectron Paramagnetic Resonance SpectroscopyCellsSpin TrapsReactive Nitrogen SpeciesReactive Oxygen SpeciesCell FunctionImmune ResponseAntioxidant Defense MechanismsOxidative DamageProtein FunctionDNA CleavageLipid PeroxidationOxidative StressCell InjuryCell DeathSuperoxide Radical Anion GenerationPeroxynitriteHydroxyl RadicalPolymorphonuclear NeutrophilsChemotactic StimulationPhorbol-12-myristate-13-acetateNicotinamide Adenine Dinucleotide Phosphate OxidaseNitric Oxide Synthase

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