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Particle Agglutination Method for Poliovirus Identification
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Particle Agglutination Method for Poliovirus Identification

Particle Agglutination Method for Poliovirus Identification

DOI:
10.3791/2824-v

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07:06 min

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April 20, 2011

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, , ,
1Department of Virology II,National Institute of Infectious Diseases, 2New Product Design Department,Fujirebio Inc.

Capítulos

  • 00:05Título
  • 01:02Introduction
  • 01:20Preparation of Anti-Poliovirus Antibodies
  • 02:49Reconstitution of PVR-IgG2a-coated Gelatin Particles
  • 03:29Particle Agglutination Assay for Poliovirus Identification
  • 05:44Identification of PV1, PV2, and PV3 by Particle Agglutination Assay
  • 06:50Conclusion

Summary

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A recently developed novel particle agglutination (PA) assay utilizing virus receptor molecule allowed a rapid and easy identification of poliovirus (PV). In this article, we will show the procedure for the PA assay for PV identification.

Tags

Particle Agglutination MethodPoliovirus IdentificationGlobal Polio Eradication InitiativeLaboratory DiagnosisPV IsolationPV IdentificationCell Culture SystemReal-time RT-PCRPost-eradication EraRapid Identification ProceduresNational LaboratoriesPA AssayPV Receptor (PVR) MoleculeSerotypes Of PVReaction PlatesAgglutination Of Gelatin Particles

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