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Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients
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JoVE Journal Medicina
Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients

Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients

DOI:
10.3791/2830-v

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13:50 min

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June 11, 2011

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, , , ,
1Department of Medicine, Surgery and Dentistry, Clinic of Infectious Diseases,San Paolo Hospital University of Milan, Italy

Capítulos

  • 00:05Título
  • 00:38Introduction
  • 03:23DNA Extraction
  • 05:16PCR
  • 07:29PCR Product Purification
  • 08:04Cloning
  • 11:21Sequencing Analysis
  • 12:44Resultados
  • 13:01Conclusion

Summary

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Our experiment will show how to perform a sequencing analysis of bacterial species translocating in peripheral blood of HIV positive patients.

Tags

SequencingBacterial MicrofloraPeripheral BloodHIV-infected PatientsGastrointestinal TractCommensal MicrobesMucosal Immune SystemMicrobiotaImmune AbnormalitiesBreach In The Gastrointestinal BarrierMucosal ImmunitySystemic CirculationBacterial BioproductsLipopolysaccharideBacterial DNA FragmentsSystemic Immune ActivationMicrobial TranslocationHIV/AIDS ImmunopathogenesisResponse To TherapyCharacterise The Composition Of Bacteria Translocating In Peripheral BloodPCR ReactionPanbacteric 16S Ribosomal GeneSequencing Analysis

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