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Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
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Capítulos
- 00:05Título
- 02:17Duck Trapping and Cloacal Swabbing
- 04:02Viral RNA Isolation and RT-PCR
- 07:28Screening for AI-positive Samples and Purification of Targeted Fragments from Gel
- 08:40Screening and Identification of PCR Products
- 09:21Representative Results
- 10:41Conclusion
A method to preserve, detect and sequence RNA from Avian Influenza Viruses was validated and extended using natural faecal samples from birds. This technique removes the necessity of maintaining a cool chain and handling of infectious viruses and can be applied in a 96-well high-throughput setup.
