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Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus

Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus

DOI:
10.3791/2834-v

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08:33 min

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July 10, 2011

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, , , ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine, 2Sue and Bill Gross Stem Cell Center,University of California, Irvine, 3Institute for Immunology,University of California, Irvine

Capítulos

  • 00:05Título
  • 01:25Preparation for Surgery and Cells for Transplant
  • 02:54Surgery and Transplantation
  • 04:45Injection of Cells
  • 07:00Sutures and Post-op Care
  • 07:53Successful Spinal Cord Transplantation
  • 08:07Conclusion

Summary

Tadução automática

Tadução automática

The transplantation of mouse neural stem cells (NSCs) into the spinal cords of mice with established demyelination is detailed. The preparation of NSCs, the laminectomy of thoracic vertebra 9 (T9), and transplantation of NSCs is outlined along with the pre- and post-operative care of the mice.

Tags

Surgical TransplantationMouse Neural Stem CellsSpinal CordsMice InfectedNeurotropic Mouse Hepatitis VirusJHM StrainDemyelinating DiseaseMultiple SclerosisTransplantation Of NSCsRemyelinationClinical OutcomeCell Replacement TherapiesChronic Neurologic DiseasesIn Vivo ModelsEngrafted CellsAdapted MethodI) Preparation Of NSCsIi) Pre-operative Care Of MiceIii) Exposure Of The Spinal Cord Via LaminectomyIv) Stereotactic Injection Of NSCsIv) Post-operative Care

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