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High-throughput gist Plasmide Overexpressie Screen
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Biologia
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JoVE Journal Biologia
High-throughput Yeast Plasmid Overexpression Screen
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High-throughput gist Plasmide Overexpressie Screen

DOI:
10.3791/2836-v

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08:57 min

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July 27, 2011

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,
1Neuroscience Graduate Group,University of Pennsylvania School of Medicine , 2Department of Cell and Developmental Biology,University of Pennsylvania School of Medicine

Capítulos

  • 00:05Título
  • 00:56Preparations for Yeast Transformation
  • 02:19Yeast Transformation Using a Liquid Handling Robot
  • 06:04Spotting Assay
  • 07:10Representative Results: Plasmid Overexpression Screen
  • 08:43Conclusion

Summary

Tadução automática

Tadução automática

Hier beschrijven we een plasmide overexpressie scherm in<em> Saccharomyces cerevisiae</em>, Met behulp van een plasmide gekleed bibliotheek en een high-throughput gist transformatie protocol met een liquid handling robot.

Tags

High-throughputYeastPlasmidOverexpression ScreenSaccharomyces CerevisiaeGenetic ScreensGene FunctionLiquid Handling RobotArrayed LibraryNeurodegenerative Disease ProteinsCellular Phenotypes

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