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Journal / Neuroscience / In vivo Electroporation of Developing Mouse Retina
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JoVE Journal Neuroscience
In vivo Electroporation of Developing Mouse Retina
 
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In vivo Electroporation of Developing Mouse Retina

Article DOI: 10.3791/2847-v • 05:54 min • June 24th, 2011
June 24th, 2011 •
1, 1,2,3,4,5
1Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, 2Department of Neurology, Johns Hopkins School of Medicine, 3Department of Ophthalmology, Johns Hopkins School of Medicine, 4Center for High-Throughput Biology, Johns Hopkins School of Medicine, 5Institute for Cell Engineering, Johns Hopkins School of Medicine

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A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical field.

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In Vivo Electroporation Developing Mouse Retina Gene Targeting Loss Of Function Knockouts Gain Of Function Experiment Cell Fate Specification Terminal Differentiation DNA Plasmids Neonatal Mouse Retina Electrical Impulses Plasma Membrane Permeability Gene Transfer Hydrophilic Plasma Membrane Pores
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