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Capítulos
- 00:05Título
- 02:14Preparation of Cells for Labeling with ReAsH/FlAsH
- 04:38Imaging the Cells on a Confocal Microscope
- 05:45Data Analysis
- 10:54Representative Assessment of the ReAsH Reactivity of TC Tagged Kinase Src in live HEK293 Cells
- 11:46Conclusion
De biarsenical kleurstoffen Flash en ReAsH binden specifiek aan tetracysteine motieven in eiwitten en kan selectief label eiwitten in levende cellen. Onlangs is dit de etikettering strategie is gebruikt om sensoren voor verschillende eiwitten conformaties of oligomere staten te ontwikkelen. Beschrijven we de etikettering van aanpak en methoden om kwantitatief te analyseren bindend.
Tags
ReAsHFlAsHLabelingImage AnalysisTetracysteine Sensor ProteinsCellsFluorescent ProteinsProtein TraffickingProtein LocalizationProtein FunctionFusion PartnersConformational ChangeProtein-associationSmall Tag SystemBiarsenical DyesSpecificityLive CellsSteric ConstraintsHost ProteinConformational Change MeasurementProtein-protein InteractionsOligomerization SensorsMutant HuntingtinAggregatesNeuronsHuntington Disease
