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单果蝇 Ommatidium解剖和影像
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JoVE Journal Neurociência
Single Drosophila Ommatidium Dissection and Imaging
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单果蝇 Ommatidium解剖和影像

DOI:
10.3791/2882-v

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05:16 min

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August 19, 2011

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, ,
1MRC Centre for Developmental Neurobiology,King’s College London

Capítulos

  • 00:05Título
  • 01:21Dissection of the Drosophila Retina
  • 02:39Dissection of the ommatidia
  • 03:26Staining and Mounting of Detached Ommatidia
  • 03:59Imaging Auto-Lysosomes in a Single Ommatidium
  • 04:43Conclusion

Summary

Tadução automática

Tadução automática

在成人使用的限制因素<em>果蝇</em>眼来研究神经退行性疾病和细胞生物学，细胞内过程的困难成像。我们描述了单小眼的解剖，产生一个真正的初级神经细胞培养，可药物治疗和先进的成像。

Tags

Drosophila MelanogasterFruit FlyNeuroscience ResearchNeurodegenerative DiseasesGenéticaFly EyeNeurodegeneration ResearchAutofluorescenceIntracellular EventsAutophagy DynamicsSingle Ommatidia DissectionDentatorubro-Pallidoluysian Atrophy (DRPLA)Technique DescriptionElectrophysiological StudiesFly Models For NeurodegenerationSubcellular Events ImagingDrug AdministrationPhotoreceptor CellsMosaic Techniques

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