Journal
/
Bioengenharia
/
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

DOI:
10.3791/2896-v

•

08:17 min

•

July 04, 2011

•

, ,
1Bioengineering Department,Northeastern University, 2Department of Pharmaceutical Sciences,Northeastern University

Capítulos

  • 00:05Título
  • 01:26Preparing the Optode Solution
  • 01:55Creating Sodium Nanosensors
  • 03:15Determining the Optimal Intracellular Nanosensor Response
  • 04:59Intracellular Injection of Nanosensors
  • 05:40Subcutaneous Injection of Nanosensors
  • 07:03Images of In Vivo and Intracellular Sodium Nanosensors
  • 07:40Conclusion

Summary

Tadução automática

Tadução automática

Fluorescent nanoparticles produced in our lab are used for imaging ion concentrations and ion fluxes in biological systems such as cells during signaling and interstitial fluid during physiological homeostasis.

Tags

Fluorescent NanoparticlesIon ConcentrationBiological SystemsIon HomeostasisDehydrationRapid Ion FluxesCellular LevelAction PotentialsSodium RegulationMonitoring Sodium LevelsIntracellular Sodium ProbesCalcium ProbesFluorescent NanosensorsSodium ConcentrationsIn VitroIn VivoIon-selective Optode TechnologyPlasticized Polymeric ParticlesSodium Specific Recognition ElementsPH-sensitive FluorophoresAdditivesMechanism Of ActionChange In FluorescenceReversible SensorsSelective For Sodium Over PotassiumDiameter Of NanoparticlesBiocompatibility

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Magnetic Resonance elastografi metode til evaluering af manipuleret væv Construct Vækst

Solid lipidnanopartikler (SLNs) til intracellulær målretning Applications

Studere Soft-stof og biologiske systemer over en bred Længde skala fra nanometer og mikrometer Størrelser på Små-vinkel Neutron diffraktometer KWS-2

Fletning Ion Koncentration Polarisering mellem sidestillede ionbyttermembraner at blokere Forplantningen Polarisering Zone

Fremstilling af metal nanopartikler ved pulserende laserablation i væsker: Et værktøj til at studere de antibakterielle egenskaber ved nanopartikler

Flash NanoPrecipitation for indkapsling af hydrofobe og hydrofile forbindelser i polymere nanopartikler

Kontinuerlig måling af biologisk støj i Escherichia Coli ved hjælp af time-lapse mikroskopi

Funktionel transkranial Doppler ultralyd til overvågning af cerebral blodgennemstrømning

Elektriske og magnetiske feltenheder til stimulering af biologisk væv

Hurtige, enzymatiske metoder til forstærkning af minimale, lineære skabeloner til proteinprototyping ved hjælp af cellefrie systemer

Read Article