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Ex-vivo-Imaging von T-Zellen in Murine Lymph Node Slices mit Weitfeld und konfokale Mikroskope
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes
DOI:

09:45 min

July 15, 2011

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Capítulos

  • 00:05Título
  • 01:18Preparing Slices from Lymph Nodes
  • 03:58Isolation and Labeling of T cells from Mouse Lymph Nodes
  • 05:08Plating Labeled T cells onto Lymph Node Slices
  • 05:45Preparation of Microscope and Lymph Node Slices for Imaging
  • 06:27Imaging T cells with a Widefield Inverted Microscope
  • 07:24Imaging T cells with a Confocal Upright Microscope
  • 07:53Representative Images and Movies of T cell Accumulation, Recruitment, and Motility within Lymph Node Slices
  • 09:17Conclusion

Summary

Tadução automática

Dieses Protokoll beschreibt eine Methode, um Bild fluoreszierenden T-Zellen in Lymphknoten Scheiben eingeführt. Die Technik ermöglicht Echtzeit-Analysen der T-Zell-Migration mit traditionellen Weitfeld-Fluoreszenz-oder konfokalen Mikroskopen.

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