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Capítulos
- 00:05Título
- 01:00Culture of Human Cord Blood-derived Endothelial Colony-forming Cells (ECFCs) and Bone-marrow Derived Mesenchymal Stem Cells (MSCs)
- 02:51Resuspension of Cells in Collagen/Fibronectin/Fibrinogen Solution (Day 0)
- 04:05Injection into Immunodeficient Nude Mouse (Day 0)
- 04:51Harvesting the Implants (Day 7) and Evaluation
- 05:50Cell Morphology
- 06:38Conclusion
Hier beschreiben wir eine Methode zur menschlichen Nabelschnurblut-derived endothelial Kolonie-bildenden Zellen (ECFCs) und Knochenmark gewonnenen mesenchymalen Stammzellen (MSCs) in einem Kollagen / Fibronektin Gel eingebettet, subkutan in immundefizienten Mäusen. Liefern Diese Zelle / Gel-Kombination erzeugt eine humane vaskuläre Netzwerk, das mit der Maus Gefäßsystem verbindet.
Tags
BioengenhariaHuman Microvascular NetworksImmunodeficient MiceCell-based TherapiesVascular NetworksIn Vivo ModelsEndothelial CellsAutologous Endothelial CellsClinical UseEndothelial Colony-forming CellsBlood-derived ECsFunctional Vascular NetworksPerivascular CellsCord Blood-derived ECFCsMesenchymal Stem Cells (MSCs)Collagen/fibronectin/fibrinogen Gel
