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Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos
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Biologia
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JoVE Journal Biologia
Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

DOI:
10.3791/307-v

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09:25 min

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October 01, 2007

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,
1Department of Neurobiology and Behaviour,University of California, Irvine (UCI)

Capítulos

  • 00:00Title and Introduction
  • 01:06Opening the Egg Window
  • 01:35Visualizing the Embryo with India Ink
  • 03:37Accessing the Embryo
  • 04:46Opening the Neural Tube
  • 05:25Placing the Growth Factor-Coated Bead
  • 08:03Closing the Egg

Summary

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A variety of growth factors and proteins interact to induce cells to take on different cell fates during development. Here we demonstrate the use of an in ovo preparation to address possible interactions between different proteins in development by placing beads on E2.5 chick embryos.

Tags

Neural TubeProteinsSpatiotemporal PatternsDevelopmentCell Fate DeterminationCell MigrationNeural CircuitsNeuronal InductionPatterningBone Morphogenetic Protein (BMP)Sonic Hedgehog (SHH)Fibroblast Growth Factor (FGF)Fgf8TelencephalonIn Ovo PreparationForebrain FormationBeads Coated With ProteinSustained ExposureMinimally InvasiveReal-time ImagingEndogenous Protein Levels

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