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Imunologia e Infecção
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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

DOI:
10.3791/3076-v

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12:50 min

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August 09, 2011

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, , ,
1St Vincent’s Institute, Department of Medicine,The University of Melbourne, 2Department of Microbiology and Immunology,The University of Melbourne

Capítulos

  • 00:05Título
  • 01:08Preparation of Listeria Inoculum and Intravenous Injection of Mice
  • 04:13Removal of Liver and Spleen from Listeria-infected Mice for Analysis
  • 05:57Preparation of Hepatic Cell Suspensions for FACS Analysis
  • 08:40Measurement of Bacterial Load in Tissues from Listeria-infected Mice
  • 10:26Representative Data for Bacterial Load and Immune Cell Analysis
  • 12:24Conclusion

Summary

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Listeria monocytogenes is a model organism for studying immune responses and genetic susceptibility to intracellular bacteria in mice. This method enables one to measure bacterial load and generate single-cell suspensions of the liver and spleen from mice for FACS analysis to determine changes in immune cells due to Listeria infection.

Tags

Bacterial LoadImmune ResponsesMiceListeria MonocytogenesGram-positiveIntracellular PathogenSystemic InfectionSpleenLiverCD8α+ Dendritic CellsKupffer CellsPhagocytosedBacterial ProteinsPhagosomeCytosolInnate Immune CellsMonocytesNeutrophilsNK CellsDendritic CellsBactericidal MechanismsCD8+ T CellsClearance Of ListeriaHost Immune ResponsesInbred Mouse StrainsGenetic Studies

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