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Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
JoVE Journal
Biologia
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JoVE Journal Biologia
Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs

Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs

DOI:
10.3791/308-v

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21:38 min

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October 01, 2007

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1Department of Physiology and Biophysics,University of California, Irvine (UCI)

Capítulos

  • 00:02Título
  • 00:06Introduction
  • 00:49Dewaxing and Rehydrating Paraffin Sections
  • 03:20Unmasking Antigen with Heat-Induced Antigen Retrieval
  • 05:14Blocking Endogenous Peroxidase Activity and Nonspecific Binding of
  • 08:17Adding Primary and Secondary Antibodies
  • 11:39Preparing and Incubating Slides in the ABC Reagent
  • 13:21Preparing and Reacting Slides with DAB Substrate Reagent
  • 16:10Counterstaining and Mounting the Slides
  • 20:46Visualizing Anti-Tyrosine Hydroxylase Slides
  • 21:07Conclusion

Tags

ImmunohistochemistryIHCParaffin SectionsVectastain ABC KitVector LabsProtein ExpressionSpecific AntibodiesDirect MethodIndirect MethodPrimary AntibodiesBiotin-conjugated Secondary AntibodiesFrozen SectionsParaffin-embedded SectionsGlass SlidesDewaxingHydrationAlcohol GradientAntigen RetrievalEndogenous Peroxidase ActivityNon-specific Binding SitesT Cell Marker CD8Tyrosine HydroxylaseSecondary Antibodies Conjugated To BiotinAvidin-conjugated Horseradish Peroxidase (HRP)Diaminiobenzidine (DAB)ContrastCoverslip

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