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生物物理检测探头相间细胞核的力学性能:基材应变的应用和微针操纵
JoVE Journal
Biologia
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JoVE Journal Biologia
Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
DOI:

16:27 min

September 14, 2011

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Capítulos

  • 00:05Título
  • 01:47Preparation of Silicone Membrane Dishes and Adherence of Cells
  • 03:30Substrate Strain Experiments
  • 06:50Substrate Strain Analysis
  • 08:35Representative Results for Substrate Strain Application on the Interphase Cell Nucleus
  • 09:57Microneedle Manipulation Experiment
  • 12:19Microneedle Manipulation Analysis
  • 13:50Representative Results for Microneedle Manipulation of the Interphase Cell Nucleus
  • 15:58Conclusion

Summary

Tadução automática

我们现在两个独立的显微镜为基础的工具来衡量,在应对全球性或局部应变的应用单一,生活贴壁细胞诱导核和细胞骨架变形。这些技术被用于确定核刚度(即变形)和探测细胞内的细胞核和细胞骨架的之间的传力。

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