/

/

测绘激光扫描Photostimulation抑制神经回路

JoVE Journal
Neurociência

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Neurociência

Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation

Please note that all translations are automatically generated. Click here for the English version.

测绘激光扫描Photostimulation抑制神经回路

DOI:

•

09:50 min

•

October 06, 2011

•

Taruna Ikrar, Nicholas D. Olivas, Yulin Shi, Xiangmin Xu²

¹Department of Anatomy and Neurobiology, School of Medicine,University of California, Irvine, ²Department of Biomedical Engineering, School of Engineering,University of California, Irvine

Capítulos

00:05Título
01:32Brain Slice Preparation
02:17Equipment Startup
03:24Whole Cell Patch-clamp Recording
05:52Laser Scanning Photostimulation
07:44Representative Results for Electrophysiological Recording and Photostimulation Mapping

Summary

Tadução automática

本文介绍了激光扫描photostimulation在表达绿色荧光蛋白在有限的抑制性神经元的人口的转基因小鼠的全细胞记录相结合的方法。该技术可以广泛的测绘和定量分析当地具体的抑制皮层神经元的突触电路。

Tags

Inhibitory Neurons Neuronal Circuits Laser Scanning Photostimulation Cortical Function Subtypes Immunochemical Properties Morphological Properties Physiological Properties Local Circuit Connections Excitatory Synaptic Input Inhibitory Synaptic Input Laser Scanning Photostimulation (LSPS)Electrical Stimulation Glutamate Puff Stimulation Mapping Resolution Cortical Circuit Analysis Whole-cell Patch Clamping

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Optogenetically有针对性的前脑电路的激光扫描光刺激

诱导星形胶质细胞受体的可塑性由神经元的放电频率操作

设计，表面处理，电镀细胞和神经元的模块化网络功能组成跨接电路的培养

激光制导神经跟踪在脑外植体

重建神经元电路：快速神经延伸和功能性神经元连接的新方法

大鼠慢性经颅电刺激及 Intracortical 记录

清醒小鼠海马毛细血管的体内纤维耦合临床前共聚焦激光扫描内窥镜（pCLE）

光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响

Optogenetic 在行为小鼠海马θ振荡中的作用

用双光子激光消融神经种群及其对斑马鱼幼虫钙成像和行为记录的评价

Read Article