Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques

Shengyuan Ding; Fu- Ming Zhou

doi:10.3791/3136

Journal

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Neurociência

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Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques

DOI:

10.3791/3136-v

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07:31 min

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September 27, 2011

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Shengyuan Ding, Fu- Ming Zhou

¹Department of Pharmacology,University of Tennessee College of Medicine

Capítulos

00:05Título
00:54Brain Slice Preparation
01:42Electrophysiological Fingerprinting of Nigral Neurons
02:48Cytoplasm Aspiration
03:29Reverse Transcription (RT) Reaction
04:23Two Stage PCR Amplification
05:14Electrophysiology and Gene Expression in Selected Neurons
07:04Conclusion

Summary

Tadução automática

Neurons are first characterized electrophysiologically. Then the cytoplasm from the recorded neuron is aspirated and subjected to reverse transcription-PCR analysis to detect the expression of mRNAs for neurotransmitter synthesis enzymes, ion channels, and receptors.

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Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques

•

•

•

Capítulos

Summary

Tadução automática

Tags

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Single-cell Profiling of Developing and Mature Retinal Neurons

Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques

Subcellular Patch-clamp Recordings from the Somatodendritic Domain of Nigral Dopamine Neurons

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