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Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques
 
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Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques

Article DOI: 10.3791/3136-v • 07:31 min • September 27th, 2011
September 27th, 2011 •
1, 1
1Department of Pharmacology, University of Tennessee College of Medicine

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Neurons are first characterized electrophysiologically. Then the cytoplasm from the recorded neuron is aspirated and subjected to reverse transcription-PCR analysis to detect the expression of mRNAs for neurotransmitter synthesis enzymes, ion channels, and receptors.

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Profiling Voltage-gated Potassium Channel MRNA Expression Nigral Neurons Single-cell RT-PCR Techniques Mammalian Central Nervous System Neurons Molecular Characteristics Functional Characteristics Morphology Gene Expression Analysis Specific Neuron Type Whole-cell Patch Clamp Recording Techniques Single-cell Reverse Transcription Polymerase Chain Reaction (scRT-PCR) MRNA Expression Profiling Substantial Nigra Electrophysiological Techniques Neurophysiological Properties Functional Properties Cytoplasm Aspiration ScRT-PCR Analysis Neurotransmitter Synthesis Enzymes Receptors Ion Channels Selectivity Sensitivity Immunohistochemistry Antibody Suitability Protein Expression Level
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