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Kwantificering en analyse van de vorming van HO-endonuclease chromosomale translocaties Gestimuleerd door Single-Strand Gloeien in Saccharomyces cerevisiae</em
JoVE Journal
Biologia
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JoVE Journal Biologia
Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
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Kwantificering en analyse van de vorming van HO-endonuclease chromosomale translocaties Gestimuleerd door Single-Strand Gloeien in Saccharomyces cerevisiae</em

DOI:
10.3791/3150-v

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09:40 min

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September 23, 2011

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, , ,
1Irell & Manella Graduate School of Biological Sciences, 2Department of Molecular and Cellular Biology,City of Hope Comprehensive Cancer Center and Beckman Research Institute, 3Department of Biochemistry and Molecular Biology,University of Southern California, Norris Comprehensive Cancer Center

Capítulos

  • 00:05Título
  • 01:16Determining HO-stimulated Translocation Frequencies
  • 02:40Determining Plating Efficiencies
  • 04:05Southern Blot Analysis
  • 04:57Chromosome Blot Analysis
  • 06:13Quantitation and Analysis of Chromosomal Translocations
  • 09:21Conclusion

Summary

Tadução automática

Tadução automática

De HO-gestimuleerde translocatie assay monitoren enkelstrengs gloeien na de oprichting van DNA dubbelstrengs breuken op meerdere loci in diploïde<em> Saccharomyces cerevisiae</em>. Dit mechanisme kan model genoom herschikkingen in somatische cellen van hogere eukaryoten na blootstelling aan hoge doses ioniserende straling.

Tags

QuantitationAnalysisFormationHO-EndonucleaseChromosomal TranslocationsSingle-Strand AnnealingSaccharomyces CerevisiaeGenetic VariationGenomic RearrangementsRepetitive ElementsEukaryotic GenomeRetrotransposon OriginLINEsSINEsExchange EventsGenome RearrangementsTranslocationsGene DosageGene ExpressionAutoimmune DiseasesCardiovascular DiseasesCancerHomologous RecombinationNon-homologous End Joining (NHEJ)Double-strand Breaks (DSBs)Mitotic CellsDNA ReplicationOxidative LesionsIonizing Radiation (IR)Exogenous DNA Damaging Agents

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