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从核衣壳的病毒DNA的制备

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Preparation of Viral DNA from Nucleocapsids

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从核衣壳的病毒DNA的制备

DOI:

•

12:45 min

•

August 16, 2011

•

Moriah L. Szpara, Yolanda R. Tafuri, L. W. Enquist

¹Department of Molecular Biology,Princeton University

Capítulos

00:05Título
01:34Day 1: Viral Infection and Preparation of Buffers
02:56Day 2, Phase 1: Cell Lysis and Ultracentrifugation
04:01Day 2, Phase 2: Ultracentrifugation
07:35“Ghost” DNA Precipitation
09:56Representative Results
11:51Conclusion

Summary

Tadução automática

我们描述了从被感染的细胞中分离高纯度的疱疹病毒衣壳DNA的过程。从溶液中捕获的是最后的DNA浓度和纯度高，使得它非常适合高通量测序，高保真PCR反应，并产生新的病毒重组体转染。

Tags

Viral DNA Nucleocapsids Host Cell Contaminants Downstream Applications Viral Genome Sequencing Host DNA Removal Viral Recombinant Strains Purified Plasmid Linear Viral DNA Extractions Density-based Centrifugation Purified Viral Capsids Cellular Proteins OD260/280 Ratio Yields Of Viral DNA Long-term Storage High-throughput Sequencing High Fidelity PCR Reactions Transfections

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