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基于乙二醇玻璃化冷冻小鼠胚胎的超低温保存
JoVE Journal
Biologia
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JoVE Journal Biologia
Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
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基于乙二醇玻璃化冷冻小鼠胚胎的超低温保存

DOI:
10.3791/3155-v

•

06:00 min

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November 18, 2011

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, , , ,
1RIKEN BioResource Center

Capítulos

  • 00:05Título
  • 01:13Vitrification of 2-cell Mouse Embryos
  • 02:22Thawing Vitrified 2-cell Mouse Embryos
  • 05:03Representative Results
  • 05:30Conclusion

Summary

Tadução automática

Tadução automática

乙二醇为基础的玻璃化冷冻方法对小鼠胚胎描述。这是有利于它的简单性和胚胎毒性低的其他方法，因此可广泛适用于小鼠多株，包括自交系和基因修饰的小鼠。

Tags

CryopreservationMouse EmbryosEthylene Glycol-based VitrificationBiomedical SciencesGenetic ModificationsSlow Freezing MethodsVitrification MethodsCryoprotectantsToxicityEmbryo DevelopmentRIKEN BioResource CenterMouse StrainsGenetic BackgroundsPhenotypesVitrification TechniqueEmbryo SurvivalCryopreservation SolutionEthylene GlycolDMSOFicollSucrose

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