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Biologia
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Kryopræservering af Mouse embryoner ethylenglycol-Based forglasning
JoVE Journal
Biologia
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JoVE Journal Biologia
Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Kryopræservering af Mouse embryoner ethylenglycol-Based forglasning

DOI:
10.3791/3155-v

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06:00 min

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November 18, 2011

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, , , ,
1RIKEN BioResource Center

Capítulos

  • 00:05Título
  • 01:13Vitrification of 2-cell Mouse Embryos
  • 02:22Thawing Vitrified 2-cell Mouse Embryos
  • 05:03Representative Results
  • 05:30Conclusion

Summary

Tadução automática

Tadução automática

En ethylen glycol-baserede forglasning metode til mus embryoner er beskrevet. Det er en fordel for andre metoder i sin enkelhed og lave embryonale toksicitet, og kan derfor være bredt anvendelige til mange stammer af mus, herunder indavlede og gen-modificerede mus.

Tags

CryopreservationMouse EmbryosEthylene Glycol-based VitrificationBiomedical SciencesGenetic ModificationsSlow Freezing MethodsVitrification MethodsCryoprotectantsToxicityEmbryo DevelopmentRIKEN BioResource CenterMouse StrainsGenetic BackgroundsPhenotypesVitrification TechniqueEmbryo SurvivalCryopreservation SolutionEthylene GlycolDMSOFicollSucrose

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