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Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
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JoVE Journal Bioengenharia
Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

DOI:
10.3791/3164-v

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08:36 min

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September 06, 2011

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, ,
1Department of Chemistry,Washington University in St. Louis

Capítulos

  • 00:05Título
  • 01:08Preparation of the Patterned Master
  • 02:30Preparation of PDMS Stamp
  • 03:11Preparation of the Patterned Gold Substrate
  • 05:08Applying Protein and Cells to the Patterned Substrate
  • 06:12Results of Creating Two-Dimentional Patterned Substrates
  • 08:06Conclusion

Summary

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Self-assembled monolayers (SAMs) formed from long chain alkane thiols on gold provide well-defined substrates for the formation of protein patterns and cell confinement. Microcontact printing of hexadecanethiol using a polydimethylsiloxane (PDMS) stamp followed by backfilling with a glycol-terminated alkane thiol monomer produces a pattern where protein and cells adsorb only to the stamped hexadecanethiol region.

Tags

Microcontact PrintingProtein ConfinementCell ConfinementWell-defined SubstratesSelf-assembled MonolayersProtein PatternsCellular StudiesPDMS StampGold-coated SubstrateAdhesive RegionsNon-adhesive RegionsHydrophobic Surface

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