/

/

蛋白质和细胞坐月子创建二维图案衬底

JoVE Journal
Bioengenharia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Bioengenharia

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Please note that all translations are automatically generated. Click here for the English version.

蛋白质和细胞坐月子创建二维图案衬底

DOI:

•

08:36 min

•

September 06, 2011

•

Dawn M. Johnson, Natalie A. LaFranzo, Joshua A. Maurer

¹Department of Chemistry,Washington University in St. Louis

Capítulos

00:05Título
01:08Preparation of the Patterned Master
02:30Preparation of PDMS Stamp
03:11Preparation of the Patterned Gold Substrate
05:08Applying Protein and Cells to the Patterned Substrate
06:12Results of Creating Two-Dimentional Patterned Substrates
08:06Conclusion

Summary

Tadução automática

金长链烷烃硫醇形成的自组装单层膜（SAMS）提供了定义良好的蛋白质模式的形成和细胞禁闭基板。使用一个邮票回填与聚二甲基硅氧烷（PDMS）微接触印刷hexadecanethiol乙二醇终止烷烃硫醇单体生产蛋白质和细胞吸附加盖hexadecanethiol地区唯一的模式。

Tags

Microcontact Printing Protein Confinement Cell Confinement Well-defined Substrates Self-assembled Monolayers Protein Patterns Cellular Studies PDMS Stamp Gold-coated Substrate Adhesive Regions Non-adhesive Regions Hydrophobic Surface

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

创建瞬态细胞膜毛孔，使用标准的喷墨打印机

创建的胶粘剂和可溶性梯度荧光显微镜成像细胞迁移

在光刻技术定义的聚对二甲苯-C细胞图案：二氧化硅<sub> 2</sub>基板

订货单细胞和单胚胎的三维约束：高内涵筛选新设备

合并一个液滴后荧光恢复来衡量磷脂单层的二维扩散

扩大奈米基材使用缝合技术的细胞行为Nanotopographical调制

使用 pDawn-Ag43 的高分辨率图案生物膜沉积

二维和三维 i 型胶原蛋白基质角质形成细胞增殖的评价

在符合标准的基质上对人类胚胎干细胞菌落的几何结构进行模式化，以控制组织级力学

使用无细胞细菌提取物、脂质体和乳液转移制备蛋白质生成合成细胞

Read Article