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Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
JoVE Journal
Biologia
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JoVE Journal Biologia
Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin

DOI:
10.3791/3187-v

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08:37 min

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November 18, 2011

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,
1Department of Physiology,Louisiana State University Health Sciences Center

Capítulos

  • 00:05Título
  • 01:27Transfection of HUVEC with GFP-Actin
  • 03:25Set up: Live-cell Imaging Chamber and Stage Heater
  • 05:20Live-cell Imaging Microscope Data Acquisition and Analysis
  • 06:14Kymograph Analysis of HUVEC
  • 08:08Conclusion

Summary

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Tadução automática

Microscopic imaging of live endothelial cells expressing GFP-actin allows characterization of dynamic changes in cytoskeletal structures. Unlike techniques that use fixed specimens, this method provides a detailed assessment of temporal changes in the actin cytoskeleton in the same cells before, during, and after various physical, pharmacological, or inflammatory stimuli.

Tags

Actin CytoskeletonEndothelial CellsGFP-ActinPermeabilityAdhesive JunctionsJunctional IntegrityCortical Actin BeltActin Stress FibersCentripetal TensionInflammatory MediatorsEndothelial PermeabilityLive-cell ImagingDynamic Nature Of Actin Structures

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