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Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats
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Biologia
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JoVE Journal Biologia
Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

DOI:
10.3791/325-v

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22:06 min

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October 01, 2007

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1Department of Physiology and Biophysics,University of California, Irvine (UCI)

Capítulos

  • 00:00Título
  • 00:06Introduction
  • 00:51Cardiac Puncture and Dissection of Thymus
  • 02:52Isolation of Antigen Presenting Cells (APCs) from Thymus
  • 06:24Activation of OVA-specific T cells by Thymic APCs
  • 12:55Adoptive Transfer of OVA-specific T cells
  • 19:27Challenging with OVA in the Ear
  • 21:02Measurment of Inflammation in the Ear
  • 21:27Conclusion

Summary

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Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T (TEM) lymphocytes. Here we demonstrate how to activate antigen-specific TEM cells, induce adoptive DTH in Lewis rats and monitor the inflammatory response.

Tags

InductionMonitoringAdoptive Delayed-type HypersensitivityRatsDTHInflammatory ReactionCCR7-Effector Memory T LymphocytesAntigenImmune SystemImmunosuppressantsCutaneous DTHAdoptive TransferActive ImmunizationIntradermal ChallengeOvalbuminTuberculinTetanus ToxoidKeyhole Limpet HemocyaninAutoantigenMyelin Basic Protein (MBP)Experimental Autoimmune Encephalomyelitis (EAE)Lewis RatsStimulationOvalbumin-specific T CellsIntraperitoneally InjectionEquilibrate In VivoPinnaSaline3-72 Hours Later

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