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使用可视化的DNA复制DNA纤维的技术，在脊椎动物的模型系统DT40
JoVE Journal
Biologia
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JoVE Journal Biologia
Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
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使用可视化的DNA复制DNA纤维的技术，在脊椎动物的模型系统DT40

DOI:
10.3791/3255-v

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07:18 min

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October 27, 2011

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1Department of Molecular Oncology, Weatherall Institute of Molecular Medicine,University of Oxford , 2Institute of Genetics and Biotechnology, Faculty of Biology,University of Warsaw

Capítulos

  • 00:05Título
  • 01:21In vivo Labeling DT40 Cells
  • 02:04Cell Lysis and DNA Spreading
  • 02:53Immunofluorescence Staining
  • 04:31Image Acquisition and Data Analysis
  • 05:24Replication Structures
  • 06:54Conclusion

Summary

Tadução automática

Tadução automática

DT40，模型脊椎动物的遗传系统，提供了强大的工具来分析蛋白质的功能。在这里，我们描述了一个简单的方法，允许在DT40细胞在单分子水平的S期DNA合成的影响参数的定性分析。

Tags

DNA ReplicationVisualizationVertebrate Model SystemDT40DNA Fiber TechniqueReplication Fork StabilityGenome DuplicationEndogenous DNA DamageExogenous DNA DamageGenomic InstabilityTumor DevelopmentFluorescence MicroscopyAvian B-cell Line DT40Protein FunctionReverse GeneticsDNA Replication And Genome StabilityReplication Fork DynamicsDNA Synthesis RateQualitative AnalysisDNA Fiber TechniqueHalogenated Nucleotides

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