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Capítulos
- 00:05Título
- 01:21In vivo Labeling DT40 Cells
- 02:04Cell Lysis and DNA Spreading
- 02:53Immunofluorescence Staining
- 04:31Image Acquisition and Data Analysis
- 05:24Replication Structures
- 06:54Conclusion
DT40, en model hvirveldyr genetiske system, giver et kraftfuldt værktøj til at analysere proteinernes funktion. Her beskriver vi en simpel metode, der giver mulighed for kvalitativ analyse af de parametre, der påvirker DNA-syntesen i S-fase i DT40 celler på enkelt molekyle niveau.
Tags
DNA ReplicationVisualizationVertebrate Model SystemDT40DNA Fiber TechniqueReplication Fork StabilityGenome DuplicationEndogenous DNA DamageExogenous DNA DamageGenomic InstabilityTumor DevelopmentFluorescence MicroscopyAvian B-cell Line DT40Protein FunctionReverse GeneticsDNA Replication And Genome StabilityReplication Fork DynamicsDNA Synthesis RateQualitative AnalysisDNA Fiber TechniqueHalogenated Nucleotides
