Journal
/
Neurociência
/
Effektiv derivering af humane Neuronal progenitorer og neuroner fra Pluripotente humane embryonale stamceller med småmolekyle Induktion
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Effektiv derivering af humane Neuronal progenitorer og neuroner fra Pluripotente humane embryonale stamceller med småmolekyle Induktion

DOI:
10.3791/3273-v

•

10:47 min

•

October 28, 2011

•

, , , , ,
1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery,Harvard Medical School, 4Division of SCI Research, VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology,Sanford-Burnham Medical Research Institute, 6La Jolla IVF

Capítulos

  • 00:05Título
  • 01:27Media Preparation and Plate Coating
  • 02:33Passaging and Seeding Undifferentiated Human ES Cells
  • 04:31Neural Induction in Adhesive Culture with Retinoic Acid
  • 05:42Differentiation in Suspension Culture and Maturation to Neuronal Phenotype after Attachment
  • 08:08Direct Neuronal Differentiation of Feeder- and Serum- Free Pluripotent Human ES Cells WIth Retinoic Acid Induction
  • 10:11Conclusion

Summary

Tadução automática

Tadução automática

Vi har etableret en protokol til induktion af neuroblasts direkte fra pluripotente menneskelige embryonale stamceller opretholdes under definerede betingelser med små molekyler, som giver udledning af et stort udbud af menneskelige neurale stamceller og neuronale celletyper i udviklingslandene CNS for neurale reparation.

Tags

Human Neuronal ProgenitorsNeuronsPluripotent Human Embryonic Stem CellsSmall Molecule InductionCell-based TherapiesCentral Nervous System (CNS)Neural Stem CellsExpansion AbilityPlasticityTrophic And Neuroprotective MoleculesPluripotent HESCsNeurological DisordersDifferentiation PotentialDevelopmental StudyClinical TranslationMulti-lineage InclinationGerm Layer

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/3273?article_type=v

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
check_url/pt/3273?article_type=v

Specifikationen af ​​Telencephalic glutamaterge neuroner fra humane pluripotente stamceller
check_url/pt/3273?article_type=v

Feeder-fri Udledning af neurale Crest stamceller fra menneskelige pluripotente stamceller
check_url/pt/3273?article_type=v

Instrueret Dopaminerg Neuron Differentiering fra menneskelige pluripotente stamceller
check_url/pt/3273?article_type=v

Udledning af leptomeninges eksplantatkulturer fra Postmortem menneskehjerne donorer
check_url/pt/3273?article_type=v

Simpel Generation af højtydende kultur af inducerede neuroner fra menneskelige voksen hud fibroblaster
check_url/pt/3273?article_type=v

Omdannelse af humane induceret pluripotente stamceller (iPSCs) til funktionel spinal og kraniel motor neuroner brug PiggyBac vektorer
check_url/pt/3273?article_type=v

Primær kultur af neuroner isoleret fra embryonale mus cerebellum
check_url/pt/3273?article_type=v

Neuronal differentiering fra mus embryonale stamceller In vitro
check_url/pt/3273?article_type=v

Afledning, ekspansion, kryopræservering og karakterisering af hjernemikrobielle endotelceller fra menneskeskabte pluripotente stamceller

Read Article