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冰帽：为发展方法拟南芥和番茄植株在96孔板高通量的基因分型
JoVE Journal
Biologia
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JoVE Journal Biologia
Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
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冰帽：为发展方法拟南芥和番茄植株在96孔板高通量的基因分型

DOI:
10.3791/3280-v

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11:15 min

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November 09, 2011

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, , , ,
1Horticulture Department,University of Wisconsin-Madison, 2Department of Zoology,Oregon State University

Capítulos

  • 00:05Título
  • 02:04Preparation of Ice-Cap Seedling Plates with Agar Growth Media
  • 02:53Sowing and Germination of Seedlings in Ice-Cap Seedling Plates
  • 03:52Transfer of Seedling Plates to the Ice-Cap Fountain
  • 05:46Harvesting of Root Tissue
  • 07:51DNA Extraction
  • 09:19Planting of Seedlings with the Desired Genotyping
  • 10:14Representative Results Illustrating Arabidopsis Seedlings Grown Using the Ice-Cap System
  • 10:36Conclusion

Summary

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Tadução automática

冰帽的方法使一个成长中的96孔板，每个幼苗根组织非破坏性的收获的植物。从这个根组织中提取的DNA可用于基因分型反应。我们已经发现，冰帽可以很好地用于<em>拟南芥</em>，番茄，水稻秧苗。

Tags

Ice-Cap MethodArabidopsisTomato Plants96-well PlatesHigh-throughput GenotypingBottleneckGenotyping PipelineHigh-throughput SolutionSeedlingsAgar Growth MediaDNA ExtractionRapid Freezing

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