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1Department of Biomedical Engineering,University of Wisconsin-Madison, 2Materials Science Program,University of Wisconsin-Madison, 3Department of Neurological Surgery,University of Wisconsin-Madison, 4Carbone Comprehensive Cancer Center and Center for Stem Cell and Regenerative Medicine,University of Wisconsin-Madison
Capítulos
- 00:05Título
- 01:57BTSC Neurosphere Dissociation
- 03:00Fabrication of Bi-layered su-8 Master
- 04:42Molding of the PDMS Stamp
- 05:31Assembly of Microfluidic Devices and Cell Culturing
- 07:04Long-term Time-lapse Imaging of BTSC Migration with BioStation IM
- 07:46Time-lapse Recordings of Migrating BTSC
- 08:40Conclusion
Un dispositif de cloisonnement microfluidique pour étudier la migration des cellules souches du cancer est décrit. Cette nouvelle plate-forme crée un microenvironnement cellulaire viable et permet la visualisation microscopique de locomotion de cellules vivantes. Très mobiles cellules cancéreuses sont isolées pour étudier les mécanismes moléculaires de l'infiltration agressive, pouvant conduire à des thérapies plus efficaces futures.
Tags
Cancer Stem Cell MigrationCompartmentalizing Microfluidic DevicesLive Cell ImagingPesquisa do CâncerCellular MigrationAggressive Cancer Cell InvasionMetastasisTherapeutic ResistanceGlioblastoma Multiforme (GBM)Primary Malignant Adult Brain TumorGBM InfiltrationTumor MicroenvironmentCancer Cell Migration MechanismsBrain Tumor Stem Cell (BTSC)Therapeutic ResistanceRecurrenceCell Migratory MechanismsConventional Migration AssaysMicrofluidic DevicesSingle Cell Analysis
