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miRNA的目标使用的任务目标ID库的全基因组屏幕
JoVE Journal
Biologia
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JoVE Journal Biologia
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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miRNA的目标使用的任务目标ID库的全基因组屏幕

DOI:
10.3791/3303-v

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08:40 min

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April 06, 2012

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, , , , ,
1Sigma-Aldrich

Capítulos

  • 00:05Título
  • 01:39Transfection with Target ID Library and Selection for Stable Cell Lines
  • 03:59Selection Reagent Kill Curves
  • 04:52Transfection of Library Cells with miRNA Expression Constructs and Selection for Stable Cell Lines and miRNA Targets
  • 06:53Library Screen for miR-373 Targets
  • 08:19Conclusion

Summary

Tadução automática

Tadução automática

目标ID图书馆是一个基于质粒，用于识别miRNA靶基因的克隆基因的全基因组的集合。在这里，我们展示它的使用和应用。

Tags

Genome-wide ScreenMiRNA TargetsMISSION Target ID LibraryPlasmid-based LibraryCDNA LibraryThymidine Kinase-zeocinStable TransformantsMiRNA Of InterestCDNAs Containing MiRNA’s TargetSequencingHuman TranscriptomeOligo-dT PrimingTotal RNASigma-Aldrich WebpageIllumina SequencingUnique GenesUCSC RefGene

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