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大肠癌细胞表面蛋白的分析，使用一种抗体的芯片和荧光多路复用

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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

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大肠癌细胞表面蛋白的分析，使用一种抗体的芯片和荧光多路复用

DOI:

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15:17 min

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September 25, 2011

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Jerry Zhou, Larissa Belov, Michael J. Solomon, Charles Chan, Stephen J. Clarke, Richard I. Christopherson

¹School of Molecular Bioscience,University of Sydney, ²Department of Surgery,Royal Prince Alfred Hospital, ³Department of Anatomical Pathology,Department of Anatomical Pathology, ⁴Department of Medicine,Concord Repatriation General Hospital

Capítulos

00:05Título
02:00Introduction
03:38Sample Digestion
04:39Sample Filtration
06:32Sample Preparation for Cell Capture
08:18Antibody Microarray Cell Capture
10:41Fluorescence Multiplexing with Conjugated Antibodies
12:38Resultados
14:37Conclusion

Summary

Tadução automática

我们描述了一个过程分解大肠直肠癌（CRC）来产生可行的单个细胞，然后在定制的抗体，认识表面抗原（DotScan CRC芯片）芯片捕获。绑定到芯片的细胞亚群可以分析使用荧光标记的单克隆抗体与荧光染料的复用。

Tags

Colorectal Cancer Cell Surface Protein Profiling Antibody Microarray Fluorescence Multiplexing CRC Prognosis Protein Expression Post-translational Modification Cluster Of Differentiation Antigens Biomarkers Immunohistochemistry (IHC)Cancer Sub-classification Prognostication Marker Clinico-pathological Staging Proteomic Techniques ITRAQ Diagnostic Laboratories

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