/
/
Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:55Isolation of Trunk Neural Tubes for Overnight Culture on Coverslips
- 06:05Loading the Modified Zigmond Chamber and Time-lapse Analysis of Cell Migration
- 09:50Representative Results
- 11:50Conclusion
An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, gentle, and capable of distinguishing chemotaxis from both chemokinesis and other influences on migratory polarity such as those derived from cell-cell interactions within the primary trunk neural crest cell culture.
