Journal
/
Biologia
/
模仿人类小分子RNA的LightSwitch萤光素酶检测系统使用3'UTR记者构造的microRNA识别目标在贴壁细胞
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3’UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
Please note that all translations are automatically generated. Click here for the English version.

模仿人类小分子RNA的LightSwitch萤光素酶检测系统使用3'UTR记者构造的microRNA识别目标在贴壁细胞

DOI:
10.3791/3343-v

•

07:19 min

•

September 28, 2011

•

, ,
1SwitchGear Genomics

Capítulos

  • 00:05Título
  • 01:20Experimental Design and GoClone 3’UTR Reported Selection
  • 02:36Co-transfection of 3′ UTR Reporters and microRNA
  • 05:00Measurement of Luciferase Activity with LightSwitch Assay Reagents
  • 05:48Luciferase Knockdown Assay: Analysis and Representative Results
  • 06:54Conclusion

Summary

Tadução automática

Tadução automática

微RNA（miRNA）是基因表达的重要调节因子，已被证明在许多生物过程中发挥的作用。为了更好地了解miRNA的UTR区的相互作用，我们已经创建了一个全基因组的3 UTR的荧光素酶记者的集合，搭配了一种新的荧光素酶基因的检测试剂，LightSwitch系统。

Tags

LightSwitch Luciferase Assay System3’UTR-reporter ConstructsMicroRNAs (miRNAs)Gene Expression RegulatorsComputational ToolsExpression AssaysProteomics AssaysChromatin-immunoprecipitation-based TechniquesMiRNA-3’UTR InteractionsFunctional EvidenceCell-based SystemGenome-wide Collection Of Human 3’UTR Luciferase ReportersSynthetic MiRNA Target Reporter ConstructsEndogenous 3’UTR Reporter ConstructsStandardized Experimental ProtocolsCo-transfectionMiRNA MimicHigh-throughput Analysis

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

研究与基于荧光素酶的记者基因检测膜生源

使用基因脉冲MXcell电穿孔系统主细胞的转染效率高

原位贴壁和非贴壁的哺乳动物细胞的亚细胞分馏

克隆形成含量：贴壁细胞

高效的推导人类心脏的前体和从多能干的小分子诱导人类胚胎干细胞的心肌细胞

人类体细胞重编程为诱导多能干细胞（iPS细胞）用绿色荧光蛋白逆转录病毒载体

对糖皮质激素信号A化学筛选程序与斑马鱼幼虫的荧光素酶报告系统

从人类体细胞与仙台病毒高效的新一代人类诱导多能干细胞

检测miRNA靶在高通量的使用3'LIFE分析

转染RAW264.7细胞用荧光素酶报告基因

Read Article