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平行板流动腔和评估内皮祖细胞层流剪应力下连续流电路

JoVE Journal
Bioengenharia

This content is Free Access.

JoVE Journal Bioengenharia

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress

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平行板流动腔和评估内皮祖细胞层流剪应力下连续流电路

DOI:

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12:43 min

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January 17, 2012

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Whitney O. Lane, Alexandra E. Jantzen, Tim A. Carlon, Ryan M. Jamiolkowski, Justin E. Grenet, Melissa M. Ley, Justin M. Haseltine, Lauren J. Galinat, Fu-Hsiung Lin, Jason D. Allen, George A. Truskey, Hardean E. Achneck

¹Department of Surgery,Duke University Medical Center, ²Department of Biomedical Engineering,Duke University , ³School of Medicine,University of Pennsylvania , ⁴Department of Medicine, Division of Cardiology,Duke University Medical Center

Capítulos

00:05Título
02:20Flow Circuit Assembly
05:30Flow Chamber Assembly
08:17Perfusate-sample Collection
09:08Evaluation of Endothelial Progenitor Cells under Laminar Flow Shear Stress
11:12Conclusion

Summary

Tadução automática

我们所描述的主题贴壁的细胞在无菌的连续流电路层流剪应力的方法。细胞的黏附，形态可通过透明腔研究，从代谢物的分析和对未来的实验或文化的剪切曝光后的干细胞的电路样本。

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