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Production and Detection of Reactive Oxygen Species (ROS) in Cancers
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Production and Detection of Reactive Oxygen Species (ROS) in Cancers

Production and Detection of Reactive Oxygen Species (ROS) in Cancers

DOI:
10.3791/3357-v

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07:17 min

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November 21, 2011

•

,
1Center for Cell and Gene Therapy,Baylor College of Medicine

Capítulos

  • 00:05Título
  • 00:46Detection of Reactive Oxygen Species Using Carboxy-H2DCFDA
  • 02:34Measuring of Nitric Oxide (NO) Production Using the Griess Assay
  • 04:42Detection of ROS Activity by Measuring Oxidized Proteins
  • 05:56Representative ROS Detection Results
  • 06:49Conclusion

Summary

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Here we propose simple methods to test and evaluate the presence of reactive oxygen species in cells.

Tags

Reactive Oxygen SpeciesROSCancersDNA DamageRNA DamageProtein OxidationLipid PeroxidationHydrogen PeroxideNitric OxideOxide AnionPeroxynitriteHydrochlorous AcidHydroxyl RadicalOxidative SpeciesPathological SituationsCellular MetabolismPhysiological SituationsCellular Signaling PathwaysOxidative And Nitrosative StressAntioxidantsSuperoxide DismutaseCatalaseGlutathione PeroxidaseGlutathioneVitamins C And EFree RadicalsImmune Responses

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