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Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model
DOI:

06:54 min

January 18, 2012

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Capítulos

  • 00:05Título
  • 01:04EVT Cells and Collagen Bead Incubation
  • 02:13Loading the Rotating Cell Culture System (RCCS)
  • 03:41Changing the Media
  • 04:37Collecting Propagated Cells
  • 05:39SGHPL-4 Trophoblast Cells in a 3D Milieu
  • 06:30Conclusion

Summary

Tadução automática

Traditional, two dimensional cell culture techniques often result in altered characteristics with respect to differentiation markers, cytokines and growth factors. Three-dimensional cell culture in the rotating cell culture system (RCCS) reestablishes expression of many of these factors as shown here with an extravillous trophoblast cell line.

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