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Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
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Neurociência
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JoVE Journal Neurociência
Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

DOI:
10.3791/3380-v

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12:00 min

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December 28, 2011

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, , , ,
1Laboratory for Perception and Memory,Institut Pasteur and Centre National de la Recherche Scientifique (CNRS)

Capítulos

  • 00:05Título
  • 01:35Preparing the Microinjector
  • 02:42Preparing the Animal and Performing the Stereotaxic Injection
  • 04:53Preparing the Miniature LED for Implantation
  • 06:54Chronic LED Implantation for in vivo Optogenetic Stimulation
  • 09:29Injected Cells and Implantation
  • 10:46Conclusion

Summary

Tadução automática

Tadução automática

Adult-born neurons of the olfactory bulb can be optogenetically controlled using Channelrhodopsin2-expressing lentiviral injection in the rostral migratory stream and chronic photostimulation with an implanted miniature LED.

Tags

Selective Viral TransductionAdult-born Olfactory NeuronsChronic In Vivo Optogenetic StimulationLocal InterneuronsAdult NeurogenesisLateral VentricleRostral Migratory Stream (RMS)Olfactory BulbLabel And Manipulate ActivityOptogenetic TechnologiesChannelrhodopsin2 (ChR2)-YFPTemporally Restricted Cohort Of NeuroblastsImplant And Calibrate Miniature LED

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